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Interaction of synthetic peptide octarphin with rat myocardium membranes

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Abstract

A selective agonist of non-opioid β-endorphin receptor synthetic peptide octarphin (TPLVTLFK, specific activity 28 Ci/mmol) was prepared. The [3H]octarphin binding to rat myocardium membranes before and after experimental myocardial infarction (EMI) was studied. It was found that [3H]octarphin with high affinity and specificity binds to non-opioid β-endorphin receptor of rat myocardium membranes before EMI: K d1 value of the [3H]octarphin specific binding to membranes was 1.8 ± 0.2 nM. In 3 h after EMI a sharp lowering in affinity of the binding is observed (K d2 = 13.3 ± 0.4 nM), and in 48 h its almost complete restoration (K d4 = 2.2 ± 0.3 nM). The results indicate participation of non-opioid β-endorphin receptor in the regulation of myocardial activity.

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Abbreviations

ECG:

electrocardiogram

EMI:

experimental myocardial infarction

References

  1. Li, C. H. (1982) Cell, 31, 504–505.

    Article  PubMed  CAS  Google Scholar 

  2. Hazum, E., Chang, K. J., and Cuatrecasas, P. (1979) Science, 205, 1033–1035.

    Article  PubMed  CAS  Google Scholar 

  3. Julliard, J. H., Shibasaki, T., Ling, N., and Guilemin, R. (1980) Science, 208, 183–185.

    Article  PubMed  CAS  Google Scholar 

  4. Houck, J. C., Kimball, C., Chang, C., Pedigo, N. W., and Yamamura, H. I. (1980) Science, 207, 78–80.

    Article  PubMed  CAS  Google Scholar 

  5. Navolotskaya, E. V., Zargarova, T. A., Malkova, N. V., Krasnova, S. B., Zav’yalov, V. P., and Lipkin, V. M. (2002) Biochem. Biophys. Res. Commun., 292, 799–804.

    Article  PubMed  CAS  Google Scholar 

  6. Navolotskaya, E. V., Kovalitskaya, Yu. A., Zolotarev, Yu. A., Kolobov, A. A., Kampe-Nemm, E. A., Malkova, N. V., Yurovskii, V. V., and Lipkin, V. M. (2004) Biochemistry (Moscow), 69, 394–400.

    Article  CAS  Google Scholar 

  7. Navolotskaya, E. V., Malkova, N. V., Lepikhova, T. N., Krasnova, S. B., Zargatova, T. A., Zav’yalov, V. P., and Lipkin, V. M. (2001) Bioorg. Khim., 27, 359–363.

    Google Scholar 

  8. Navolotskaya, E. V., Zargarova, T. A., Malkova, N. V., Lepikhova, T. N., Zav’yalov, V. P., and Lipkin, V. M. (2001) Peptides, 22, 2009–2013.

    Article  PubMed  CAS  Google Scholar 

  9. Navolotskaya, E. V., Malkova, N. V., Zargarova, T. A., Krasnova, S. B., and Lipkin, V. M. (2002) Biochemistry (Moscow), 67, 357–363.

    Article  CAS  Google Scholar 

  10. Navolotskaya, E. V., Kolobov, A. A., Kampe-Nemm, E. A., Zargarova, T. A., Malkova, N. V., Krasnova, S. B., Kovalitskaya, Yu. A., Zav’yalov, V. P., and Lipkin, V. M. (2003) Biochemistry (Moscow), 68, 34–41.

    Article  CAS  Google Scholar 

  11. Navolotskaya, E. V., Zargarova, T. A., Malkova, N. V., Zharmukhamedova, T. Yu., Kolobov, A. A., Kampe-Nemm, E. A., Yurovsky, V. V., and Lipkin, V. M. (2003) Biochem. Biophys. Res. Commun., 303, 1065–1072.

    Article  PubMed  CAS  Google Scholar 

  12. Navolotskaya, E. V., Kovalitskaya, E. V., Zolotarev, Yu. A., Kudryashova, N. Yu., Goncharenko, E. N., Kolobov, A. A., Kampe-Nemm, E. A., Malkova, N. V., Yurovskii, V. V., and Lipkin, V. M. (2004) Biochemistry (Moscow), 69, 870–875.

    Article  CAS  Google Scholar 

  13. Navolotskaya, E. V., Kovalitskaya, Y. A., Zolotarev, Y. A., and Sadovnikov, V. B. (2008) J. Peptide Sci., 14, 1121–1128.

    Article  CAS  Google Scholar 

  14. Nekrasova, Y. N., Sadovnikov, V. B., Zolotarev, Y. A., and Navolotskaya, E. B. (2010) J. Peptide Sci., 16, 263–268.

    CAS  Google Scholar 

  15. Kovalitskaya, Yu. A., Nekrasova, Yu. N., Sadovnikov, V. B., Zolotarev, Yu. A., and Navolotskaya, E. V. (2011) Biochemistry (Moscow), 76, 596–604.

    Article  CAS  Google Scholar 

  16. Schnolzer, M., Alewood, P., Jones, A., Alewood, D., and Kent, S. B. H. (1992) Int. J. Peptide Protein Res., 40, 180–193.

    Article  CAS  Google Scholar 

  17. Zolotarev, Yu. A., Dadayan, A. K., Bocharov, E. V., Borisov, Yu. A., Vaskovsky, B. V., Dorokhova, E. M., and Myasoedov, N. F. (2003) Amino Acids, 24, 325–333.

    Article  PubMed  CAS  Google Scholar 

  18. Dal Farra, C., Zsurger, N., Vincent, J.-P., and Cupo, A. (2000) Peptides, 21, 577–587.

    Article  Google Scholar 

  19. Lowry, O. H., Rosebrough, N. J., Farr, O. L., and Randal, R. J. (1951) J. Biol. Chem., 193, 265–275.

    PubMed  CAS  Google Scholar 

  20. Pennock, B. E. (1973) Anal. Biochem., 56, 306–309.

    Article  PubMed  CAS  Google Scholar 

  21. Chang, Y. C., and Prusoff, W. H. (1973) Biochem. Pharmacol., 22, 3099–3108.

    Article  Google Scholar 

  22. Barron, B. A. (1999) Cardiovasc. Res., 43, 13–16.

    Article  PubMed  CAS  Google Scholar 

  23. Barron, B. A. (2000) Proc. Soc. Exp. Biol. Med., 224, 1–7.

    Article  PubMed  CAS  Google Scholar 

  24. Pepe, S., van den Brink, O. W., Lakatta, E. G., and Xiao, R. P. (2004) Cardiovasc. Res., 63, 414–422.

    Article  PubMed  CAS  Google Scholar 

  25. Millington, W. R., Rosenthal, D. W., Unal, C. B., and Nyquist-Battie, C. (1999) Cardiovasc. Res., 43, 107–116.

    Article  PubMed  CAS  Google Scholar 

  26. Oldroyd, K. G., Hrvey, K., Gray, C. E., Beastall, G. H., and Cobbe, S. M. (1992) Br. Heart J., 67, 230–235.

    Article  PubMed  CAS  Google Scholar 

  27. Chang, M. C., Lee, A. Y., Lin, W. Y., Chen, T. J., Shyu, M. Y., and Chang, W. F. (2004) Jpn. Heart J., 45, 365–371.

    Article  PubMed  CAS  Google Scholar 

  28. Bolte, C., Newman, G., and Schultz, J. (2009) Am. J. Physiol. Heart Circ. Physiol., 296, 967–975.

    Article  Google Scholar 

  29. Bolte, C., Newman, G., and Schultz, J. (2009) J. Mol. Cell Cardiol., 47, 493–503.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, 142290, Pushchino, Moscow Region, Russia

    Y. N. Nekrasova & E. V. Navolotskaya

  2. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, 123182, Moscow, Russia

    Y. A. Zolotarev

Authors
  1. Y. N. Nekrasova
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  2. Y. A. Zolotarev
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  3. E. V. Navolotskaya
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Corresponding author

Correspondence to E. V. Navolotskaya.

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Original Russian Text © Y. N. Nekrasova, Y. A. Zolotarev, E. V. Navolotskaya, 2011, published in Biokhimiya, 2011, Vol. 76, No. 12, pp. 1659–1664.

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Nekrasova, Y.N., Zolotarev, Y.A. & Navolotskaya, E.V. Interaction of synthetic peptide octarphin with rat myocardium membranes. Biochemistry Moscow 76, 1337–1341 (2011). https://doi.org/10.1134/S0006297911120066

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  • DOI: https://doi.org/10.1134/S0006297911120066

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