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Analysis of the dinoflagellate Prorocentrum minimum transcriptome: Identifying the members of the voltage-gated cation channel superfamily

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Abstract

Dinoflagellates are an ecologically important group of aquatic single-cell eukaryotes. At present, relatively little is known about physiological features that determine the role of these protists in natural ecosystems. The lack of knowledge on the diversity, structure, and functioning of dinoflagellate ion channels significantly hampers the interpretation of physiological reactions and adaptations in these microorganisms. We performed the analysis of the translated transcriptome databases that belong to two strains of the dinoflagellate Prorocentrum minimum in order to identify the members of the voltage-gated cation channel superfamily. We found out that transcriptomes of these potentially toxic microorganisms contained the homologues of: 1—inwardly rectifying potassium channels (Kir), 2—voltage-gated potassium channels (Kv), 3—calciumactivated potassium channels (KCa), 4—cyclic nucleotide-gated channels (EAG and HCN/CNG), 5—TRPV and TRPP channels, 6—two-pore calcium channels TPC, 7—voltage-gated sodium (Nav) and calcium (Cav) channels, 8—and voltage-gated proton channels (Hv).

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Abbreviations

a. a.:

amino acid residues

Ank:

ankyrin domain

BK:

large conductance calcium-activated potassium channel

Cav :

Cav—voltage-gated calcium channel

CNBD:

cyclic nucleotide-binding domain

CNG:

cyclic nucleotide-gated channel

EAG:

ether-a-go-go like channel

HCN:

hyperpo-larization-activated cyclic nucleotide-gated channel

K2P :

potassium leak channel

Kir :

inwardly rectifying potassium channel

Kv :

voltage-gated potassium channel

NALCN:

sodium leak channel

Nav :

voltage-gated sodium channel

P-loop:

pore loop

VSD:

voltage-sensitive domain

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Authors and Affiliations

  1. Institute of Cytology RAS, St. Petersburg, 194064, Russia

    I. A. Pozdnyakov & S. O. Skarlato

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  1. I. A. Pozdnyakov
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  2. S. O. Skarlato
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Correspondence to I. A. Pozdnyakov.

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Original Russian Text © I.A. Pozdnyakov, S.O. Skarlato, 2015, published in Tsitologiya, 2015, Vol. 57, No. 7, pp. 533–543.

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Pozdnyakov, I.A., Skarlato, S.O. Analysis of the dinoflagellate Prorocentrum minimum transcriptome: Identifying the members of the voltage-gated cation channel superfamily. Cell Tiss. Biol. 9, 483–492 (2015). https://doi.org/10.1134/S1990519X15060085

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