This Article Full Text Full Text (PDF) Other Versions of this Article: JVI.00962-07v1 82/5/2575    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Google Scholar Articles by Anderson, J. L. Articles by Hope, T. J. PubMed PubMed Citation Articles by Anderson, J. L. Articles by Hope, T. J.

 Previous Article  |  Next Article 

Journal of Virology, March 2008, p. 2575-2579, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.00962-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Heat Shock Perturbs TRIM5 Restriction of Human Immunodeficiency Virus Type 1

Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611

Received 3 May 2007/ Accepted 28 November 2007

TRIM5 restriction factors protect target cells from retroviruses by blocking infection prior to the accumulation of viral reverse transcription (RT) products. Here, we demonstrate that heat shock perturbed owl monkey TRIMCyp and rhesus TRIM5-mediated restriction of human immunodeficiency virus type 1 (HIV-1) late RT products and 2-long terminal repeat circles. Heat shock partially rescued HIV-1 infection from TRIMCyp restriction, and this rescue became more profound when combined with the presence of the proteasome inhibitor MG132. This indicates that viral RT products rescued from restriction by either heat shock treatment or the presence of MG132 are on a productive pathway, supporting a model in which TRIM5 proteins restrict retroviruses in multiple phases that are differentially sensitive to heat shock and proteasome inhibitors.

Published ahead of print on 12 December 2007.