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Journal of Clinical Microbiology, July 2005, p. 3255-3259, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3255-3259.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA

USDA-ARS, Animal Disease Research Unit, Pullman, Washington 99164,¹ College of Veterinary Medicine, Washington State University, Pullman, Washington 99164,² USDA-ARS Microbial Genomics and Bioprocessing Research Unit, Peoria, Illinois 61604,³ Genisphere, Inc., Hatfield, Pennsylvania 19440⁴

Received 23 February 2005/ Returned for modification 4 April 2005/ Accepted 6 April 2005

Listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. Suspension microarray technology eliminates the technical issues associated with planar microarrays and allows high-throughput subtyping of L. monocytogenes strains. In this study, a suspension array assay using dendrimer signal amplification allowed rapid and accurate serovar identification of L. monocytogenes strains using genomic DNA as a target. The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many different regions within the bacterial genome and should allow high-resolution subtyping not possible with multiplex PCR.

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