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Journal of Clinical Microbiology, July 2005, p. 3198-3202, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3198-3202.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genotyping of Methicillin-Resistant Staphylococcus aureus Strains from Two Hospitals in Bangalore, South India

Gayathri Arakere,1* Savitha Nadig,1 Göte Swedberg,2 Ragini Macaden,3 Satish K. Amarnath,4 and Dasarathy Raghunath1

Sir Dorabji Tata Centre for Research in Tropical Diseases, Bangalore, India,1 Department of Medical Biochemistry and Microbiology, University of Uppsala, Uppsala, Sweden,2 St. John's Medical College Hospital, Bangalore, India,3 Department of Microbiology, Manipal Hospital, Bangalore, India4

Received 25 November 2004/ Returned for modification 21 January 2005/ Accepted 23 March 2005

Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in India, and up to 70% methicillin resistance has been reported from hospitals in various parts of India. Hospitals use phenotyping for the most part, and molecular genotyping is not done. Here we report on the genotyping of 82 single-patient isolates from two hospitals in Bangalore, South India, for the first time. Most of the strains possessed type III or IIIA staphylococcal cassette chromosome (SCCmec) cassettes, and we did not detect strains with type I, IA, or II cassettes. Most isolates also contained the type III cassette chromosome recombinase (ccr) AB region. Multilocus sequence typing (MLST) and staphylococcal protein A (spa) typing of a selected number of isolates have been carried out. Although most isolates that were chosen for MLST and spa typing had the same patterns, they were quite diverse in their pulsed-field gel electrophoresis (PFGE) patterns. PFGE, MLST, and spa typing of the Indian strains revealed that they are related to the previously described Hungarian and Brazilian clones.

* Corresponding author. Mailing address: Sir Dorabji Tata Centre for Research in Tropical Diseases, Society for Innovation and Development, IISc Campus, Malleswaram, Bangalore 560012, India. Phone: 91-80-23466007. Fax: 91-80-23466006. E-mail: garakere{at}hotmail.com.

Journal of Clinical Microbiology, July 2005, p. 3198-3202, Vol. 43, No. 7
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.7.3198-3202.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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