JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JCM.02302-07v1
46/4/1491    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Casson, N.
Right arrow Articles by Greub, G.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casson, N.
Right arrow Articles by Greub, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2008, p. 1491-1493, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02302-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples{triangledown}

Nicola Casson,1 Klara M. Posfay-Barbe,2 Alain Gervaix,2 and Gilbert Greub1*

Center for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland,1 Children's Hospital of Geneva, University Hospitals of Geneva, Geneva, Switzerland2

Received 30 November 2007/ Returned for modification 10 January 2008/ Accepted 18 January 2008

Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

* Corresponding author. Mailing address: Center for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne 1011, Switzerland. Phone: 0041-21-314 49 79. Fax: 0041-21-314 40 60. E-mail: gilbert.greub{at}chuv.ch

{triangledown} Published ahead of print on 30 January 2008.

Journal of Clinical Microbiology, April 2008, p. 1491-1493, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02302-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2008 by the American Society for Microbiology. All rights reserved.