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Journal of Bacteriology, January 2005, p. 576-592, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.576-592.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates{dagger}

James E. Cassat,1 Paul M. Dunman,2 Fionnuala McAleese,2 Ellen Murphy,2 Steven J. Projan,3 and Mark S. Smeltzer1*

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas,1 Wyeth Research, Pearl River, New York,2 Wyeth Research, Cambridge, Massachusetts3

Received 19 April 2004/ Accepted 5 October 2004

Much of the research aimed at defining the pathogenesis of Staphylococcus aureus has been done with a limited number of strains, most notably the 8325-4 derivative RN6390. Several lines of evidence indicate that this strain is unique by comparison to clinical isolates of S. aureus. Based on this, we have focused our efforts on two clinical isolates (UAMS-1 and UAMS-601), both of which are hypervirulent in our animal models of musculoskeletal infection. In this study, we used comparative genomic hybridization to assess the genome content of these two isolates relative to RN6390 and each of seven sequenced S. aureus isolates. Our comparisons were done by using an amplicon-based microarray from the Pathogen Functional Genomics Resource Center and an Affymetrix GeneChip that collectively represent the genomes of all seven sequenced strains. Our results confirmed that UAMS-1 and UAMS-601 share specific attributes that distinguish them from RN6390. Potentially important differences included the presence of cna and the absence of isaB, sarT, sarU, and sasG in the UAMS isolates. Among the sequenced strains, the UAMS isolates were most closely related to the dominant European clone EMRSA-16. In contrast, RN6390, NCTC 8325, and COL formed a distinct cluster that, by comparison to the other four sequenced strains (Mu50, N315, MW2, and SANGER-476), was the most distantly related to the UAMS isolates and EMRSA-16.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, mail slot 511, University of Arkansas for Medical Sciences, 4301 W. Markham, Little Rock, AR 72205. Phone: (501) 686-7958. Fax: (501) 686-5359. E-mail: smeltzermarks{at}uams.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, January 2005, p. 576-592, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.576-592.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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