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Journal of Bacteriology, March 2007, p. 1589-1596, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01151-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

PduL Is an Evolutionarily Distinct Phosphotransacylase Involved in B₁₂-Dependent 1,2-Propanediol Degradation by Salmonella enterica Serovar Typhimurium LT2

Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011,¹ Foundation for Applied Molecular Evolution, Gainesville, Florida 32601,² Department of Pediatrics, University of Florida, Gainesville, Florida 32611,³ Department of Pediatrics, Section of Oncology and Hematology, Baylor College of Medicine, Houston, Texas 77030,⁴ Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611⁵

Received 28 July 2006/ Accepted 3 December 2006

Salmonella enterica degrades 1,2-propanediol (1,2-PD) in a coenzyme B₁₂-dependent manner. Previous enzymatic assays of crude cell extracts indicated that a phosphotransacylase (PTAC) was needed for this process, but the enzyme involved was not identified. Here, we show that the pduL gene encodes an evolutionarily distinct PTAC used for 1,2-PD degradation. Growth tests showed that pduL mutants were unable to ferment 1,2-PD and were also impaired for aerobic growth on this compound. Enzyme assays showed that cell extracts from a pduL mutant lacked measurable PTAC activity in a background that also carried a pta mutation (the pta gene was previously shown to encode a PTAC enzyme). Ectopic expression of pduL corrected the growth defects of a pta mutant. PduL fused to eight C-terminal histidine residues (PduL-His₈) was purified, and its kinetic constants were determined: the V_max was 51.7 ± 7.6 µmol min^–1 mg^–1, and the K_m values for propionyl-PO₄^2– and acetyl-PO₄^2– were 0.61 and 0.97 mM, respectively. Sequence analyses showed that PduL is unrelated in amino acid sequence to known PTAC enzymes and that PduL homologues are distributed among at least 49 bacterial species but are absent from the Archaea and Eukarya.

Published ahead of print on 8 December 2006.

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