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Journal of Bacteriology, September 2006, p. 6680-6687, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00605-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression of the Primary Carbohydrate Component of the Bordetella bronchiseptica Biofilm Matrix Is Dependent on Growth Phase but Independent of Bvg Regulation

Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6084,¹ Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada²

Received 28 April 2006/ Accepted 2 July 2006

We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix was xylose, and linkage analysis indicated a majority of it to be in a 4-linked polymeric form. The production of xylose was independent of Bvg regulation but instead was dependent on bacterial growth phase. In addition, N-acetyl-glucosamine in the matrix was found to be important for the initial development of the biofilm. These results suggest that B. bronchiseptica biofilm formation is growth phase dependent in addition to being regulated by the Bvg virulence system.

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