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Journal of Bacteriology, May 2008, p. 3155-3160, Vol. 190, No. 9
0021-9193/08/$08.00+0     doi:10.1128/JB.00053-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic,¹ Centre of Biostatistics and Analyses, Faculty of Science and Faculty of Medicine, Masaryk University, Kamenice 126/3, 625 00 Brno, Czech Republic²

Received 11 January 2008/ Accepted 15 February 2008

In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

Published ahead of print on 29 February 2008.

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