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Infection and Immunity, July 2005, p. 4414-4419, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4414-4419.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inhibition of Proteolytic Activity of a Chlamydial Proteasome/Protease-Like Activity Factor by Antibodies from Humans Infected with Chlamydia trachomatis

Jyotika Sharma, Feng Dong, Mustak Pirbhai, and Guangming Zhong*

Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, Texas 78229

Received 23 January 2005/ Returned for modification 4 February 2005/ Accepted 12 February 2005

We have previously shown that individuals infected with Chlamydia trachomatis can develop a robust antibody response to a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor [CPAF]). We now report that sera collected from these infected individuals neutralized the proteolytic activity of CPAF. Depletion of the serum sample with CPAF proteins to remove the CPAF-specific antibodies effectively blocked the neutralization, whereas similar depletion with the HSP60 proteins failed to do so. We further demonstrated that the CPAF central region covering residues 200 to 338 was predominantly recognized by the human neutralization antibodies. The significance of the CPAF neutralization antibodies generated in chlamydia-infected individuals is discussed.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229. Phone: (210) 567-1169. Fax: (210) 567-0293. E-mail: zhongg{at}uthscsa.edu.

Editor: J. D. Clements

Infection and Immunity, July 2005, p. 4414-4419, Vol. 73, No. 7
0019-9567/05/$08.00+0     doi:10.1128/IAI.73.7.4414-4419.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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