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Infection and Immunity, October 2004, p. 6148-6150, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.6148-6150.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Protective Effect of a Tuberculosis Subunit Vaccine Based on a Fusion of Antigen 85B and ESAT-6 in the Aerosol Guinea Pig Model

Anja W. Olsen,¹ Ann Williams,² Limei M. Okkels,^1, Graham Hatch,² and Peter Andersen^1*

Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark,¹ Research Division, Health Protection Agency, Porton Down, Salisbury, United Kingdom²

Received 24 March 2004/ Returned for modification 21 May 2004/ Accepted 13 July 2004

A fusion protein of antigen 85B (Ag85B) and ESAT-6 administered in cationic lipid vesicles conferred a highly significant level of protection against Mycobacterium tuberculosis in the guinea pig aerosol model of infection. The protection was manifested as delayed clinical illness and prolonged survival. Neither Ag85B nor ESAT-6 (independently or as a cocktail) induced significant protection in this model.

Editor: J. D. Clements

Present address: Core Facility Protein Analysis, Max Planck Institute for Infection Biology, Berlin, Germany.

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