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Infection and Immunity, August 2002, p. 4581-4590, Vol. 70, No. 8
0019-9567/02/$04.00+0     DOI: 10.1128/IAI.70.8.4581-4590.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Helicobacter pylori-Induced Activation of Human Endothelial Cells

Department of Medical Microbiology and Immunology, Sahlgrenska Academy at Göteborg University, Göteborg,¹ Department of Bacteriology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden,³ Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, Paris, France²

Received 23 October 2001/ Returned for modification 11 January 2002/ Accepted 18 April 2002

Helicobacter pylori infection causes active chronic inflammation with a continuous recruitment of neutrophils to the inflamed gastric mucosa. To evaluate the role of endothelial cells in this process, we have examined adhesion molecule expression and chemokine and cytokine production from human umbilical vein endothelial cells stimulated with well-characterized H. pylori strains as well as purified proteins. Our results indicate that endothelial cells actively contribute to neutrophil recruitment, since stimulation with H. pylori bacteria induced upregulation of the adhesion molecules VCAM-1, ICAM-1, and E-selectin as well as the chemokines interleukin 8 (IL-8) and growth-related oncogene alpha (GRO-) and the cytokine IL-6. However, there were large variations in the ability of the different H. pylori strains to stimulate endothelial cells. These interstrain variations were seen irrespective of whether the strains had been isolated from patients with duodenal ulcer disease or asymptomatic carriers and were not solely related to the expression of known virulence factors, such as the cytotoxin-associated gene pathogenicity island, vacuolating toxin A, and Lewis blood group antigens. In addition, one or several unidentified proteins which act via NF-B activation seem to induce endothelial cell activation. In conclusion, human endothelial cells produce neutrophil-recruiting factors and show increased adhesion molecule expression after stimulation with certain H. pylori strains. These effects probably contribute to the continuous recruitment of neutrophils to H. pylori-infected gastric mucosa and may also contribute to tissue damage and ulcer formation.

Editor: R. N. Moore

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