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Infection and Immunity, June 2006, p. 3488-3497, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.02006-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Major Subunit, CfaB, of Colonization Factor Antigen I from Enterotoxigenic Escherichia coli Is a Glycosphingolipid Binding Protein

Lena Jansson,1 Joshua Tobias,2 Michael Lebens,2 Ann-Mari Svennerholm,2 and Susann Teneberg1*

Departments of Medical Biochemistry,1 Medical Microbiology and Immunology, Institute of Biomedicine, Göteborg University, S-405 30 Göteborg, Sweden2

Received 13 December 2005/ Returned for modification 16 February 2006/ Accepted 30 March 2006

Bacterial adherence to mucosal surfaces is an important virulence trait of pathogenic bacteria. Adhesion of enterotoxigenic Escherichia coli (ETEC) to the intestine is mediated by a number of antigenically distinct colonization factors (CFs). One of the most common CFs is CFA/I. This has a fimbrial structure composed of a major repeating subunit, CfaB, and a single tip subunit, CfaE. The potential carbohydrate recognition by CFA/I was investigated by binding CFA/I-fimbriated bacteria and purified CFA/I fimbriae to a large number of variant glycosphingolipids separated on thin-layer chromatograms. For both fimbriated bacteria and purified fimbriae, specific interactions could be identified with a number of nonacid glycosphingolipids. These included glucosylceramide, lactosylceramide with phytosphingosine and/or hydroxy fatty acids, neolactotetraosylceramide, gangliotriaosylceramide, gangliotetraosylceramide, the H5 type 2 pentaglycosylceramide, the Lea-5 glycosphingolipid, the Lex-5 glycosphingolipid, and the Ley-6 glycosphingolipid. These glycosphingolipids were also recognized by recombinant E. coli expressing CFA/I in the absence of tip protein CfaE, as well as by purified fimbriae from the same strain. This demonstrates that the glycosphingolipid-binding capacity of CFA/I resides in the major CfaB subunit.

* Corresponding author. Mailing address: Institute of Biomedicine, Göteborg University, P.O. Box 440, S-405 30 Göteborg, Sweden. Phone: 46 31 773 34 92. Fax: 46 31 413 190. E-mail: Susann.Teneberg{at}medkem.gu.se.

Editor: A. D. O'Brien

Infection and Immunity, June 2006, p. 3488-3497, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.02006-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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