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Infection and Immunity, October 2007, p. 4885-4890, Vol. 75, No. 10
0019-9567/07/$08.00+0     doi:10.1128/IAI.01942-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Implication of Quorum Sensing in Salmonella enterica Serovar Typhimurium Virulence: the luxS Gene Is Necessary for Expression of Genes in Pathogenicity Island 1{triangledown}

Jeongjoon Choi, Dongwoo Shin,{dagger} and Sangryeol Ryu*

Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Republic of Korea

Received 11 December 2006/ Returned for modification 5 April 2007/ Accepted 29 June 2007

Despite the fact that the regulatory system sensing density of cell population and its signaling molecule have been identified in Salmonella enterica, the biological significance of this phenomenon termed as quorum sensing remains unknown. In this report, we provide evidence that the luxS gene is necessary for Salmonella virulence phenotypes. Transcription assays showed that the cell-density-dependent induction of the invF gene was abolished in a Salmonella strain with the luxS gene deleted. The effect of the luxS deletion was also investigated in other InvF-regulated genes expressed from Salmonella pathogenicity island 1 (SPI-1). The decreased expression of SPI-1 genes in the strain with luxS deleted could be restored by either the addition of a synthetic signal molecule or the introduction of a plasmid copy of the luxS gene. Thus, the reduced expression of invF and its regulated genes in Salmonella cells lacking quorum sensing resulted in the attenuation of virulence phenotypes both in vitro and in vivo.

* Corresponding author. Mailing address: Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea. Phone: 82-2-880-4856. Fax: 82-2-873-5095. E-mail: sangryu{at}snu.ac.kr

{triangledown} Published ahead of print on 9 July 2007.

Editor: V. J. DiRita

{dagger} Present address: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.

Infection and Immunity, October 2007, p. 4885-4890, Vol. 75, No. 10
0019-9567/07/$08.00+0     doi:10.1128/IAI.01942-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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