IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hasenstein, J. R.
Right arrow Articles by Lamont, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hasenstein, J. R.
Right arrow Articles by Lamont, S. J.

 Previous Article  |  Next Article 

Infection and Immunity, June 2006, p. 3375-3380, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.00027-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analyses of Five Gallinacin Genes and the Salmonella enterica Serovar Enteritidis Response in Poultry

Jason R. Hasenstein,1 Guolong Zhang,2 and Susan J. Lamont1*

Department of Animal Science, Iowa State University, Ames, Iowa 50011,1 Department of Animal Science, Oklahoma State University, Stillwater, Oklahoma 740782

Received 5 January 2006/ Returned for modification 6 February 2006/ Accepted 6 March 2006

Gallinacins in poultry are functional equivalents of mammalian beta-defensins, which constitute an integral component of the innate immune system. Salmonella enterica serovar Enteritidis is a gram-negative bacterium that negatively affects both human and animal health. To analyze the association of genetic variations of the gallinacin genes with the phenotypic response to S. enterica serovar Enteritidis, an F1 population of chickens was created by crossing four outbred broiler sires to dams of two highly inbred lines. The F1 chicks were evaluated for bacterial colonization after pathogenic S. enterica serovar Enteritidis inoculation and for circulating antibody levels after inoculation with S. enterica serovar Enteritidis bacterin vaccine. Five candidate genes were studied, including gallinacins 2, 3, 4, 5, and 7. Gene fragments were sequenced from the founder individuals of the resource population, and a mean of 13.2 single-nucleotide polymorphisms (SNP) per kilobase was identified. One allele-defining SNP per gene was utilized to test for statistical associations of sire alleles with progeny response to S. enterica serovar Enteritidis. Among the five gallinacin genes evaluated, the Gal3 and Gal7 SNPs in broiler sires were found to be associated with antibody production after S. enterica serovar Enteritidis vaccination. Utilization of these SNPs as molecular markers for the response to S. enterica serovar Enteritidis may result in the enhancement of the immune response in poultry.

* Corresponding author. Mailing address: Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, IA 50011-3150. Phone: (515) 294-4100. Fax: (515) 294-2401. E-mail: sjlamont{at}iastate.edu.

Editor: F. C. Fang

Infection and Immunity, June 2006, p. 3375-3380, Vol. 74, No. 6
0019-9567/06/$08.00+0     doi:10.1128/IAI.00027-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2006 by the American Society for Microbiology. All rights reserved.