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Clinical and Vaccine Immunology, May 2007, p. 641-643, Vol. 14, No. 5
1071-412X/07/$08.00+0     doi:10.1128/CVI.00474-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Recombinant Polypeptide Antigen-Based Immunoglobulin G Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Dengue{triangledown}

Flavia B. Dos Santos,1* Rita Maria R. Nogueira,1 Monique R. Q. Lima,1 Thatiane S. De Simone,1 Hermann G. Schatzmayr,1 Elezer M. B. Lemes,2 Eva Harris,3 and Marize P. Miagostovich1

Department of Virology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21045-360, Brazil,1 Department of Technological Development, Bio-Manguinhos, Rio de Janeiro 21045-360, Brazil,2 Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720-73603

Received 18 December 2006/ Returned for modification 8 February 2007/ Accepted 8 March 2007

We have developed an indirect enzyme-linked immunosorbent assay for detection of anti-dengue virus (DENV) immunoglobulin G antibodies using four recombinant DENV envelope polypeptides as antigens, which demonstrated a sensitivity of 89.4% and a specificity of 93.3%. These easily produced antigens are a feasible, cost-effective alternative for generating reagents for dengue serological tests.

* Corresponding author. Mailing address: Flavivirus Laboratory-Department of Virology, Oswaldo Cruz Institute/FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro 21045-360, Brazil. Phone and fax: 55(21) 2598-4373. E-mail: flaviab{at}ioc.fiocruz.br

{triangledown} Published ahead of print on 28 March 2007.

Clinical and Vaccine Immunology, May 2007, p. 641-643, Vol. 14, No. 5
1071-412X/07/$08.00+0     doi:10.1128/CVI.00474-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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