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Applied and Environmental Microbiology, September 2005, p. 5590-5592, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5590-5592.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food

Kim E. Sapsford,1 Chris R. Taitt,2 Nicole Loo,2 and Frances S. Ligler2*

George Mason University, 10910 University Blvd., MS 4E3, Manassas, Virginia 20110,1 Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-54382

Received 4 November 2004/ Accepted 29 March 2005

Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml and 20 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.

* Corresponding author. Mailing address: Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5438. Phone: (202) 404-6002. Fax: (202) 404-8897. E-mail: fligler{at}cbmse.nrl.navy.mil.

Applied and Environmental Microbiology, September 2005, p. 5590-5592, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5590-5592.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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