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Applied and Environmental Microbiology, January 2005, p. 547-549, Vol. 71, No. 1
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.1.547-549.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Scarce Evidence of Yogurt Lactic Acid Bacteria in Human Feces after Daily Yogurt Consumption by Healthy Volunteers
Rosa del Campo,1*
Daniel Bravo,1
Rafael Cantón,1
Patricia Ruiz-Garbajosa,1
Raimundo García-Albiach,2
Alejandra Montesi-Libois,2
Francisco-Javier Yuste,1
Victor Abraira,1 and
Fernando Baquero1
Departments of Microbiology, Preventive Medicine, and Biostatistics, Ramón y Cajal University Hospital,1
Department of Microbiology, San Pablo-CEU University, Madrid, Spain2
Received 3 June 2004/
Accepted 24 August 2004
In a double-blind prospective study including 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and Streptococcus thermophilus after repeated yogurt consumption (15 days) was analyzed by culture, specific PCR, and DNA hybridization of total fecal DNA. Detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative. DNA compatible with yogurt bacteria was found by hybridization experiments in only 10 (10.52%) of 96 individuals after consumption of fresh yogurt and in 2 (2.10%) of 96 individuals after consumption of pasteurized yogurt (P = 0.01).
* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra. Colmenar, Km 9.1, Madrid 28.034, Spain. Phone: 34-91 3368542. Fax: 34-91 3368809. E-mail: rosacampo{at}yahoo.com.
Applied and Environmental Microbiology, January 2005, p. 547-549, Vol. 71, No. 1
0099-2240/05/$08.00+0 doi:10.1128/AEM.71.1.547-549.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.