AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hay, A. G.
Right arrow Articles by Sayler, G. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hay, A. G.
Right arrow Articles by Sayler, G. S.
Agricola
Right arrow Articles by Hay, A. G.
Right arrow Articles by Sayler, G. S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2000, p. 4589-4594, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Bioluminescent Whole-Cell Reporter for Detection of 2,4-Dichlorophenoxyacetic Acid and 2,4-Dichlorophenol in Soil

Anthony G. Hay,dagger James F. Rice,Dagger Bruce M. Applegate, Nathan G. Bright, and Gary S. Sayler*

Center for Environmental Biotechnology, University of Tennessee, Knoxville, Knoxville, Tennessee 37996-1605

Received 1 May 2000/Accepted 25 June 2000

A bioreporter was made containing a tfdRPDII-luxCDABE fusion in a modified mini-Tn5 construct. When it was introduced into the chromosome of Ralstonia eutropha JMP134, the resulting strain, JMP134-32, produced a sensitive bioluminescent response to 2,4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 2.0 µM to 5.0 mM. This response was linear (R2 = 0.9825) in the range of 2.0 µM to 1.1 × 102 µM. Saturation occurred at higher concentrations, with maximal bioluminescence occurring in the presence of approximately 1.2 mM 2,4-D. A sensitive response was also recorded in the presence of 2,4-dichlorophenol at concentrations below 1.1 × 102 µM; however, only a limited bioluminescent response was recorded in the presence of 3-chlorobenzoic acid at concentrations below 1.0 mM. A significant bioluminescent response was also recorded when strain JMP134-32 was incubated with soils containing aged 2,4-D residues.

* Corresponding author. Mailing address: Center for Environmental Biotechnology, 676 Dabney Hall, University of Tennessee, Knoxville, Knoxville, TN 37996-1605. Phone: (423) 974-8080. Fax: (423) 974-8086. E-mail: Sayler{at}utk.edu.

dagger Present address: Department of Microbiology, Cornell University, Ithaca, NY 14853.

Dagger Present address: Monsanto Company, St. Louis, MO 63167-0001.

Applied and Environmental Microbiology, October 2000, p. 4589-4594, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.