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Applied and Environmental Microbiology, April 2008, p. 2275-2287, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02646-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cell Invasion and Matricide during Photorhabdus luminescens Transmission by Heterorhabditis bacteriophora Nematodes{triangledown} ,{dagger}

Todd A. Ciche,1* Kwi-suk Kim,1 Bettina Kaufmann-Daszczuk,1 Ken C. Q. Nguyen,2 and David H. Hall2

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824,1 The Center for C. elegans Anatomy, Albert Einstein College of Medicine, Bronx, New York 104622

Received 21 November 2007/ Accepted 8 February 2008

Many animals and plants have symbiotic relationships with beneficial bacteria. Experimentally tractable models are necessary to understand the processes involved in the selective transmission of symbiotic bacteria. One such model is the transmission of the insect-pathogenic bacterial symbionts Photorhabdus spp. by Heterorhabditis bacteriophora infective juvenile (IJ)-stage nematodes. By observing egg-laying behavior and IJ development, it was determined that IJs develop exclusively via intrauterine hatching and matricide (i.e., endotokia matricida). By transiently exposing nematodes to fluorescently labeled symbionts, it was determined that symbionts infect the maternal intestine as a biofilm and then invade and breach the rectal gland epithelium, becoming available to the IJ offspring developing in the pseudocoelom. Cell- and stage-specific infection occurs again in the pre-IJ pharyngeal intestinal valve cells, which helps symbionts to persist as IJs develop and move to a new host. Synchronous with nematode development are changes in symbiont and host behavior (e.g., adherence versus invasion). Thus, Photorhabdus symbionts are maternally transmitted by an elaborate infectious process involving multiple selective steps in order to achieve symbiont-specific transmission.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824. Phone: (517) 355-6463. Fax: (517) 355-8957. E-mail: ciche{at}msu.edu

{triangledown} Published ahead of print on 15 February 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, April 2008, p. 2275-2287, Vol. 74, No. 8
0099-2240/08/$08.00+0     doi:10.1128/AEM.02646-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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