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Applied and Environmental Microbiology, February 2008, p. 916-919, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01557-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Determination of the Shear Force at the Balance between Bacterial Attachment and Detachment in Weak-Adherence Systems, Using a Flow Displacement Chamber{triangledown}

M. Reza Nejadnik,1 Henny C. van der Mei,1* Henk J. Busscher,1 and Willem Norde1,2

Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands,1 Laboratory of Physical Chemistry and Colloid Science, Wageningen University, Dreijenplein 6, 6703 HB Wageningen, The Netherlands2

Received 10 July 2007/ Accepted 26 November 2007

We introduce a procedure for determining shear forces at the balance between attachment and detachment of bacteria under flow. This procedure can be applied to derive adhesion forces in weak-adherence systems, such as polymer brush coatings, which are currently at the center of attention for their control of bacterial adhesion and biofilm formation.

* Corresponding author. Mailing address: Department of Biomedical Engineering (Sector F), University Medical Center Groningen and University of Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands. Phone: 31-50-3633140. Fax: 31-50-3633159. E-mail: h.c.van.der.mei{at}med.umcg.nl

{triangledown} Published ahead of print on 7 December 2007.

Applied and Environmental Microbiology, February 2008, p. 916-919, Vol. 74, No. 3
0099-2240/08/$08.00+0     doi:10.1128/AEM.01557-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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