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Antimicrobial Agents and Chemotherapy, June 2002, p. 2041-2045, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.2041-2045.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Lentivirus Lytic Peptide 1 Perturbs both Outer and Inner Membranes of Serratia marcescens

Shruti M. Phadke,¹ Vanja Lazarevic,² Caroline C. Bahr,² Kazi Islam,² Donna Beer Stolz,³ Simon Watkins,³ Sarah B. Tencza,^2, Hans J. Vogel,⁴ Ronald C. Montelaro,² and Timothy A. Mietzner^2*

Department of Pediatrics, Children's Hospital of Pittsburgh,¹ Department of Molecular Genetics & Biochemistry,² Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261,³ Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada⁴

Received 5 November 2001/ Returned for modification 8 January 2002/ Accepted 19 February 2002

Bis-lentivirus lytic protein 1 (Bis-LLP1) and polymyxin B exhibited similar killing activities against Serratia marcescens. By electron microscopy, bis-LLP1 interacted with the outer and cytoplasmic bacterial membranes, while polymyxin B affected only the outer membrane. The results of standard biochemical probes supported the findings of the electron microscopy studies, suggesting that these antimicrobial peptides have different mechanisms of action.

Present address: Cellomics, Inc., Pittsburgh, PA 15219.

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