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Journal of Bacteriology, July 2005, p. 4627-4636, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4627-4636.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genomic Sequence of an Otitis Media Isolate of Nontypeable Haemophilus influenzae: Comparative Study with H. influenzae Serotype d, Strain KW20

Alistair Harrison,1 David W. Dyer,2 Allison Gillaspy,2 William C. Ray,1 Rachna Mungur,1 Matthew B. Carson,2 Huachun Zhong,1 Jenny Gipson,2 Mandy Gipson,2 Linda S. Johnson,1 Lisa Lewis,2 Lauren O. Bakaletz,1 and Robert S. Munson Jr.1*

Center for Microbial Pathogenesis, Columbus Children's Research Institute, and Department of Pediatrics, Ohio State University College of Medicine and Public Health, Columbus, Ohio 43205,1 Laboratory for Genomics and Bioinformatics, and Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 731042

Received 11 January 2005/ Accepted 29 March 2005

In 1995, the Institute for Genomic Research completed the genome sequence of a rough derivative of Haemophilus influenzae serotype d, strain KW20. Although extremely useful in understanding the basic biology of H. influenzae, these data have not provided significant insight into disease caused by nontypeable H. influenzae, as serotype d strains are not pathogens. In contrast, strains of nontypeable H. influenzae are the primary pathogens of chronic and recurrent otitis media in children. In addition, these organisms have an important role in acute otitis media in children as well as other respiratory diseases. Such strains must therefore contain a gene repertoire that differs from that of strain Rd. Elucidation of the differences between these genomes will thus provide insight into the pathogenic mechanisms of nontypeable H. influenzae. The genome of a representative nontypeable H. influenzae strain, 86-028NP, isolated from a patient with chronic otitis media was therefore sequenced and annotated. Despite large regions of synteny with the strain Rd genome, there are large rearrangements in strain 86-028NP's genome architecture relative to the strain Rd genome. A genomic island similar to an island originally identified in H. influenzae type b is present in the strain 86-028NP genome, while the mu-like phage present in the strain Rd genome is absent from the strain 86-028NP genome. Two hundred eighty open reading frames were identified in the strain 86-028NP genome that were absent from the strain Rd genome. These data provide new insight that complements and extends the ongoing analysis of nontypeable H. influenzae virulence determinants.

* Corresponding author. Mailing address: Columbus Children's Research Institute, Center for Microbial Pathogenesis, 700 Children's Drive, Columbus, OH 43205-2696. Phone: (614) 722-2778. Fax: (614) 722-2818. E-mail: munsonr{at}pediatrics.ohio-state.edu.

Journal of Bacteriology, July 2005, p. 4627-4636, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4627-4636.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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