JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An authors' corrections has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chain, P.
Right arrow Articles by Arp, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chain, P.
Right arrow Articles by Arp, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2003, p. 2759-2773, Vol. 185, No. 9
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.9.2759-2773.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Complete Genome Sequence of the Ammonia-Oxidizing Bacterium and Obligate Chemolithoautotroph Nitrosomonas europaea{dagger}

Patrick Chain,1,2 Jane Lamerdin,1,2 Frank Larimer,1,3 Warren Regala,1,2 Victoria Lao,1,2 Miriam Land,1,3 Loren Hauser,1,3 Alan Hooper,4 Martin Klotz,5 Jeanette Norton,6 Luis Sayavedra-Soto,7 Dave Arciero,4 Norman Hommes,7 Mark Whittaker,4 and Daniel Arp7*

Joint Genome Institute, Walnut Creek, California 94598,1 Lawrence Livermore National Laboratory, Livermore, California 94550,2 Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831,3 University of Minnesota, Minneapolis, Minnesota 55455,4 University of Louisville, Louisville, Kentucky 40208,5 Utah State University, Logan, Utah 84322,6 Oregon State University, Corvallis, Oregon 973317

Received 21 November 2002/ Accepted 20 February 2003

Nitrosomonas europaea (ATCC 19718) is a gram-negative obligate chemolithoautotroph that can derive all its energy and reductant for growth from the oxidation of ammonia to nitrite. Nitrosomonas europaea participates in the biogeochemical N cycle in the process of nitrification. Its genome consists of a single circular chromosome of 2,812,094 bp. The GC skew analysis indicates that the genome is divided into two unequal replichores. Genes are distributed evenly around the genome, with ~47% transcribed from one strand and ~53% transcribed from the complementary strand. A total of 2,460 protein-encoding genes emerged from the modeling effort, averaging 1,011 bp in length, with intergenic regions averaging 117 bp. Genes necessary for the catabolism of ammonia, energy and reductant generation, biosynthesis, and CO2 and NH3 assimilation were identified. In contrast, genes for catabolism of organic compounds are limited. Genes encoding transporters for inorganic ions were plentiful, whereas genes encoding transporters for organic molecules were scant. Complex repetitive elements constitute ca. 5% of the genome. Among these are 85 predicted insertion sequence elements in eight different families. The strategy of N. europaea to accumulate Fe from the environment involves several classes of Fe receptors with more than 20 genes devoted to these receptors. However, genes for the synthesis of only one siderophore, citrate, were identified in the genome. This genome has provided new insights into the growth and metabolism of ammonia-oxidizing bacteria.

* Corresponding author. Mailing address: Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331-2902. Phone: (541) 737-1294. Fax: (541) 737-5310. E-mail: arpd{at}bcc.orst.edu.

{dagger} For a commentary on this article, see page 2690 in this issue.

Journal of Bacteriology, May 2003, p. 2759-2773, Vol. 185, No. 9
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.9.2759-2773.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 2003 by the American Society for Microbiology. All rights reserved.