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Journal of Bacteriology, July 2000, p. 4124-4127, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

All Major Regions of FtsK Are Required for Resolution of Chromosome Dimers

David S. Boyle,* Dawn Grant, G. Craig Draper,dagger and William D. Donachie

Institute of Cell & Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland

Received 20 December 1999/Accepted 13 April 2000

Resolution of chromosome dimers, by site-specific recombination between dif sites, is carried out in Escherichia coli by XerCD recombinase in association with the FtsK protein. We show here that a variety of altered FtsK polypeptides, consisting of the N-terminal (cell division) domain alone or with deletions in the proline-glutamine-rich part of the protein, or polypeptides consisting of the C-terminal domain alone are all unable to carry out dif recombination. Alteration of the putative nucleotide-binding site also abolishes the ability of FtsK to carry out recombination between dif sites.

* Corresponding author. Present address: Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121. Phone: (858) 450-5990, ext. 257. Fax: (858) 550-3998. E-mail: Dboyle{at}skcc.org.

dagger Present address: Department of Chemistry & Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095.

Journal of Bacteriology, July 2000, p. 4124-4127, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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