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Applied and Environmental Microbiology, March 2005, p. 1462-1472, Vol. 71, No. 3
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.3.1462-1472.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Functional Characterization of Two Cytochrome P450 Monooxygenase Genes, P450-1 and P450-4, of the Gibberellic Acid Gene Cluster in Fusarium proliferatum (Gibberella fujikuroi MP-D)

S. Malonek,^1, M. C. Rojas,² P. Hedden,³ P. Gaskin,³ P. Hopkins,³ and B. Tudzynski^1*

Westfälische Wilhelms-Universität Münster, Institut für Botanik, Münster, Germany,¹ Laboratorio de Bioorgánica, Departamento de Química, Facultad de Ciencias, Universidad de Chile, Santiago, Chile,² Rothamsted Research, Harpenden, Herts, United Kingdom³

Received 29 June 2004/ Accepted 8 October 2004

Gibberella fujikuroi is a species complex with at least nine different biological species, termed mating populations (MPs) A to I (MP-A to MP-I), known to produce many different secondary metabolites. So far, gibberellin (GA) production is restricted to Fusarium fujikuroi (G. fujikuroi MP-C), although at least five other MPs contain all biosynthetic genes. Here, we analyze the GA gene cluster and GA pathway in the closest related species, Fusarium proliferatum (MP-D), and demonstrate that the GA genes share a high degree of sequence homology with the corresponding genes of MP-C. The GA production capacity was restored after integration of the entire GA gene cluster from MP-C, indicating the existence of an active regulation system in F. proliferatum. The results further indicate that one reason for the loss of GA production is the accumulation of several mutations in the coding and 5' noncoding regions of the ent-kaurene oxidase gene, P450-4.

Present address: Dep. Plant-Microbe Interactions, Max-Planck-Institute for Breeding Research, 50829 Cologne, Germany.

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