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Science 3 March 2000:
Vol. 287. no. 5458, pp. 1615 - 1622
DOI: 10.1126/science.287.5458.1615
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Research Articles

The Catalytic Pathway of Cytochrome P450cam at Atomic Resolution

Ilme Schlichting, 1* Joel Berendzen, 2 Kelvin Chu, 2dagger Ann M. Stock, 3 Shelley A. Maves, 4 David E. Benson, 4 Robert M. Sweet, 5 Dagmar Ringe, 6 Gregory A. Petsko, 6 Stephen G. Sligar 14

Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature--a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.

1 Max Planck Institute for Molecular Physiology, Department of Physical Biochemistry, Otto Hahn Strasse 11, 44227 Dortmund, Germany.
2 Biophysics Group, Mail Stop D454, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
3 Center for Advanced Biotechnology and Medicine, 679 Joes Lane, Piscataway, NJ 08854-5638, USA.
4 Beckman Institute, University of Illinois, 405 N. Mathews, Urbana, IL 61801, USA.
5 Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA.
6 Rosenstiel Center, Brandeis University, 415 South Street, Waltham, MA 02254-9110, USA.
*   To whom correspondence should be addressed. E-mail: ilme.schlichting{at}mpi-dortmund.mpg.de

dagger    Present address: Department of Physics, Cook Building, University of Vermont, Burlington, VT 05405-0125, USA.

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   Abstract »    Full Text »    PDF »
Probing the open state of cytochrome P450cam with ruthenium-linker substrates.
A. R. Dunn, I. J. Dmochowski, A. M. Bilwes, H. B. Gray, and B. R. Crane (2001)
PNAS 98, 12420-12425
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