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Science 14 May 1999:
Vol. 284. no. 5417, pp. 1180 - 1183
DOI: 10.1126/science.284.5417.1180
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Reports

Mammalian Transgenesis by Intracytoplasmic Sperm Injection

Anthony C. F. Perry, 1* Teruhiko Wakayama, 1 Hidefumi Kishikawa, 1 Tsuyoshi Kasai, 1 Masaru Okabe, 2 Yutaka Toyoda, 3 Ryuzo Yanagimachi 1

Coinjection of unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta -galactosidase reporter produced 64 to 94 percent transgene-expressing embryos, reflecting DNA-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos in the GFP series generated about 20 percent offspring expressing the integrated transgene. These data indicate that exogenous DNA can reproducibly be delivered into an oocyte by microinjected spermatozoa and suggest an adaptable method of transgenesis.

1 Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, HI 96822, USA.
2 Genome Information Research Center, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan.
3 The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080, Japan.
*   To whom correspondence should be addressed. E-mail: perry{at}hawaii.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)