Visualization of Specific B and T Lymphocyte Interactions in the Lymph Node
Paul Garside,
*
Elizabeth Ingulli,
*
Rebecca
R. Merica,
Julia G. Johnson,
Randolph J. Noelle,
Marc K. Jenkins
Early events in the humoral immune response were visualized in
lymph nodes by simultaneous tracking of antigen-specific CD4 T and B
cells after immunization. The T cells were initially activated in the T
cell areas when the B cells were still randomly dispersed in the B
cell-rich follicles. Both populations then migrated to the edges of
the follicles and interacted there, resulting in CD154-dependent B cell
proliferation and germinal center formation. These results provide
visual documentation of cognate T-B cell interactions and localize them
to the follicular border.
P. Garside, University of Glasgow, Department of Immunology,
Western Infirmary, Glasgow, G11 6NT, United Kingdom. E. Ingulli,
Department of Pediatrics, University of Minnesota Medical School,
Minneapolis, MN 55455, USA. R. R. Merica, J. G. Johnson,
M. K. Jenkins, Department of Microbiology and the Center for
Immunology at the University of Minneota Medical School, Minneapolis,
MN 55455, USA. R. J. Noelle, Dartmouth Medical College, Department
of Microbiology, Lebanon, NH, USA.
*
These authors contributed equally to this work.
To whom correspondence should be addressed.
