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Science 27 March 1998:
Vol. 279. no. 5359, pp. 2118 - 2121
DOI: 10.1126/science.279.5359.2118
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Reports

Monoallelic Expression of the Interleukin-2 Locus

Georg A. Holländer, * Saulius Zuklys, dagger Corinne Morel, dagger Emiko Mizoguchi, Kathrine Mobisson, Stephen Simpson, ddagger Cox Terhorst, William Wishart, David E. Golan, Atul K. Bhan, Steven J. Burakoff *

The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. Uncontrolled secretion of IL-2 results in adverse reactions ranging from anergy, to aberrant T cell activation, to autoimmunity. With the use of fluorescent in situ hybridization and single-cell polymerase chain reaction in cells with different IL-2 alleles, IL-2 expression in mature thymocytes and T cells was found to be tightly controlled by monoallelic expression. Because IL-2 is encoded at a nonimprinted autosomal locus, this result represents an unusual regulatory mode for controlling the precise expression of a single gene.

G. A. Holländer, Pediatric Immunology, Department of Research and Children's Hospital, Basel University Medical School, 4031 Basel, Switzerland, and Division of Pediatric Oncology, Dana Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
S. Zuklys, Department of Research and Children's Hospital, Basel University Medical School, 4031 Basel, Switzerland.
C. Morel and W. Wishart, Preclinical Research, Building 360, Novartis Pharma, 4002 Basel, Switzerland.
E. Mizoguchi and A. K. Bhan, Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USA.
K. Mobisson and S. J. Burakoff, Division of Pediatric Oncology, Dana Farber Cancer Institute, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
S. Simpson and C. Terhorst, Division of Immunology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02115, USA.
D. E. Golan, Departments of Biological Chemistry and Molecular Pharmacology and of Medicine, Harvard Medical School and Division of Hematology-Oncology, Brigham and Women's Hospital, Boston, MA 02115, USA.
*   To whom correspondence should be addressed.

dagger    Both authors contributed equally to this work.

ddagger    Present address: Education and Research Center, St. Vincent Hospital, Elm Park, Dublin, Ireland.

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