Probing Single Secretory Vesicles with Capillary Electrophoresis

doi:10.1126/science.279.5354.1190

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Science 20 February 1998:
Vol. 279. no. 5354, pp. 1190 - 1193
DOI: 10.1126/science.279.5354.1190

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Reports

Probing Single Secretory Vesicles with Capillary Electrophoresis

Daniel T. Chiu, Sheri J. Lillard, Richard H. Scheller, Richard N. Zare, ^* Sandra E. Rodriguez-Cruz, Evan R. Williams, Owe Orwar, Mats Sandberg, J. Anders Lundqvist

Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individuallywith a combination of optical trapping, capillary electrophoresisseparation, and a laser-induced fluorescence detection. With theuse of optical trapping, a single vesicle that had attoliters(10¹⁸ liters) of volume was introduced into the tapered inlet of aseparation capillary. Once the vesicle was injected, it was lysed,and its components were fluorescently labeled with naphthalene-2,3-dicarboxaldehydebefore separation. The resultant electropherograms indicated distinctvariations in the contents of single vesicles.

D. T. Chiu, S. J. Lillard, R. N. Zare, Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
R. H. Scheller, Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
S. E. Rodriguez-Cruz and E. R. Williams, Department of Chemistry, University of California, Berkeley, CA 94720, USA.
O. Orwar and J. A. Lundqvist, Department of Chemistry, Göteborg University, SE-412 96, Göteborg, Sweden.
M. Sandberg, Department of Anatomy and Cell Biology, Göteborg University, SE-413 90, Göteborg, Sweden.
^* To whom correspondence should be addressed.