CD1d-Restricted and TCR-Mediated Activation of V{alpha}14 NKT Cells by Glycosylceramides

doi:10.1126/science.278.5343.1626

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Science 28 November 1997:
Vol. 278. no. 5343, pp. 1626 - 1629
DOI: 10.1126/science.278.5343.1626

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Reports

CD1d-Restricted and TCR-Mediated Activation of V14 NKT Cells by Glycosylceramides

Tetsu Kawano, Junqing Cui, Yasuhiko Koezuka, Isao Toura, Yoshikatsu Kaneko, Kazuhiro Motoki, Hitomi Ueno, Ryusuke Nakagawa, Hiroshi Sato, Eisuke Kondo, Haruhiko Koseki, Masaru Taniguchi ^*

Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the V14 and J281gene segments. A glycosylceramide-containing $alpha$ -anomeric sugarwith a longer fatty acyl chain (C₂₆) and sphingosine base (C₁₈)was identified as a ligand for this TCR. Glycosylceramide-mediatedproliferative responses of V14 NKT cells were abrogated bytreatment with chloroquine-concanamycin A or by monoclonal antibodiesagainst CD1d/V8, CD40/CD40L, or B7/CTLA-4/CD28, but not byinterference with the function of a transporter-associated protein.Thus, this lymphocyte shares distinct recognition systems witheither T or NK cells.

T. Kawano, J. Cui, I. Toura, Y. Kaneko, H. Sato, E. Kondo, H. Koseki, M. Taniguchi, CREST (Core Research for Evolutional Science and Technology) Project, Japan Science and Technology Corporation (JST), and Division of Molecular Immunology, Center for Biomedical Science, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260, Japan.
Y. Koezuka, K. Motoki, H. Ueno, R. Nakagawa, Pharmaceutical Research Laboratory, Kirin Brewery, 3 Miyahara, Takasaki, Gunma 370-12, Japan.
^* To whom correspondence should be addressed. E-mail: taniguti{at}med.m.chiba-u.ac.jp