In Vivo Gene Delivery and Stable Transduction of Nondividing
Cells by a Lentiviral Vector
Luigi Naldini,
Ulrike Blömer,
Philippe Gallay,
Daniel Ory,
Richard Mulligan,
Fred H. Gage,
Inder M. Verma,
*
Didier Trono
A retroviral vector system based on the human immunodeficiency
virus (HIV) was developed that, in contrast to a murine leukemia
virus-based counterpart, transduced heterologous sequences
into HeLa cells and rat fibroblasts blocked in the cell cycle, as well
as into human primary macrophages. Additionally, the HIV vector could
mediate stable in vivo gene transfer into terminally differentiated
neurons. The ability of HIV-based viral vectors to deliver genes in
vivo into nondividing cells could increase the applicability of
retroviral vectors in human gene therapy.
L. Naldini, U. Blömer, P. Gallay, F. H. Gage, I. M. Verma,
D. Trono, Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA
92037, USA.
D. Ory and R. Mulligan, Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
*
To whom correspondence should be addressed.
