From the Genome to the Proteome: Uncovering Peptides in the Apis Brain

doi:10.1126/science.1124128

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Science 27 October 2006:
Vol. 314. no. 5799, pp. 647 - 649
DOI: 10.1126/science.1124128

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From the Genome to the Proteome: Uncovering Peptides in the Apis Brain

Amanda B. Hummon,¹ Timothy A. Richmond,¹ Peter Verleyen,² Geert Baggerman,² Jurgen Huybrechts,² Michael A. Ewing,¹ Evy Vierstraete,² Sandra L. Rodriguez-Zas,³^,4^,5^* Liliane Schoofs,²^* Gene E. Robinson,⁴^,5^,6^,7^* Jonathan V. Sweedler¹^,4^,5^,7^*

Neuropeptides, critical brain peptides that modulate animalbehavior by affecting the activity of almost every neuronalcircuit, are inherently difficult to predict directly from anascent genome sequence because of extensive posttranslationalprocessing. The combination of bioinformatics and proteomicsallows unprecedented neuropeptide discovery from an unannotatedgenome. Within the Apis mellifera genome, we have inferred morethan 200 neuropeptides and have confirmed the sequences of 100peptides. This study lays the groundwork for future molecularstudies of Apis neuropeptides with the identification of 36genes, 33 of which were previously unreported.

¹ Department of Chemistry, University of Illinois, Urbana, IL 61801, USA.
³ Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
⁴ Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA.
⁵ Neuroscience Program, University of Illinois, Urbana, IL 61801, USA.
⁶ Department of Entomology, University of Illinois, Urbana, IL 61801, USA.
² Laboratory of Developmental Physiology, Genomics and Proteomics, Katholieke Universiteit Leuven, Naamsestraat 59, Louvain B-3000, Belgium.
⁷ Beckman Institute, University of Illinois, Urbana, IL 61801, USA.

^* To whom correspondence should be addressed: jsweedle{at}uiuc.edu

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