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Science 4 February 2005:
Vol. 307. no. 5710, pp. 724 - 727
DOI: 10.1126/science.1105103
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Reports

Dynamic Complex Formation During the Yeast Cell Cycle

Ulrik de Lichtenberg,1* Lars Juhl Jensen,2* Søren Brunak,1 Peer Bork2,3{dagger}

To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.

1 Center for Biological Sequence Analysis, Technical University of Denmark, DK-2800 Lyngby, Denmark.
2 European Molecular Biology Laboratory, D-69117 Heidelberg, Germany.
3 Max-Delbrück-Centre for Molecular Medicine, D-13092 Berlin, Germany.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: bork{at}embl.de

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Science. ISSN 0036-8075 (print), 1095-9203 (online)