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Originally published in Science Express on 3 January 2002
Science 8 February 2002:
Vol. 295. no. 5557, pp. 1089 - 1092
DOI: 10.1126/science.1068228
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Reports

Production of alpha -1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

Liangxue Lai,1 Donna Kolber-Simonds,3 Kwang-Wook Park,1 Hee-Tae Cheong,14 Julia L. Greenstein,3 Gi-Sun Im,15 Melissa Samuel,1 Aaron Bonk,1 August Rieke,1 Billy N. Day,1 Clifton N. Murphy,1 David B. Carter,12 Robert J. Hawley,3 Randall S. Prather1*

The presence of galactose alpha -1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha -1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

1 Department of Animal Science,
2 Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
3 Immerge BioTherapeutics Inc., Charlestown, MA 02129, USA.
4 Department of Veterinary Medicine, College of Animal Resource Science, Kangwon National University, Chunchon 200-701, Korea.
5 National Livestock Research Institute, Suwon 441-350, Korea.
*   To whom correspondence should be addressed. E-mail: pratherR{at}missouri.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)