Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Orchestrated Transcription of Key Pathways in Arabidopsis by the Circadian Clock
Stacey L. Harmer,1John B. Hogenesch,2Marty Straume,3Hur-Song Chang,4Bin Han,4Tong Zhu,4Xun Wang,4Joel A. Kreps,4Steve A. Kay12*
Like most organisms, plants have endogenous biological
clocks that coordinate internal events with the external environment.We used high-density oligonucleotide microarrays to examine geneexpression in Arabidopsis and found that 6% of the more
than 8000genes on the array exhibited circadian changes in
steady-statemessenger RNA levels. Clusters of circadian-regulated
genes werefound in pathways involved in plant responses to light and
otherkey metabolic pathways. Computational analysis of cycling genesallowed the identification of a highly conserved promoter motifthat we
found to be required for circadian control of gene expression.Our
study presents a comprehensive view of the temporal
compartmentalizationof physiological pathways by the circadian clock
in a eukaryote.
1 Department of Cell Biology, Scripps Research
Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 Genomics Institute of the Novartis Research
Foundation, 3115 Merryfield Row, La Jolla, CA 92121, USA.
3 Center for Biomathematical Technology, NSF Center
for Biological Timing, Division of Endocrinology and Metabolism,
Department of Internal Medicine, University of Virginia,
Charlottesville, VA 22904, USA.
4 Novartis
Agricultural Discovery Institute, 3115 Merryfield Row, San Diego, CA
92121, USA.
*
To whom correspondence should be addressed. E-mail:
stevek{at}scripps.edu
The editors suggest the following Related Resources on Science sites:
In Science Magazine
NEWS FOCUS
Elizabeth Pennisi (15 December 2000) Science290 (5499), 2054.
[DOI: 10.1126/science.290.5499.2054] |Summary »|Full Text »
NEWS FOCUS
Elizabeth Pennisi (15 December 2000) Science290 (5499), 2055.
[DOI: 10.1126/science.290.5499.2055] |Summary »|Full Text »
NEWS FOCUS
Elizabeth Pennisi (15 December 2000) Science290 (5499), 2056.
[DOI: 10.1126/science.290.5499.2056] |Summary »|Full Text »
NEWS FOCUS
Christine Mlot (15 December 2000) Science290 (5499), 2057.
[DOI: 10.1126/science.290.5499.2057] |Summary »|Full Text »
EDITORIAL
Caroline Dean (15 December 2000) Science290 (5499), 2071.
[DOI: 10.1126/science.290.5499.2071] |Summary »
POLICY FORUM
Chris Somerville and Jeff Dangl (15 December 2000) Science290 (5499), 2077.
[DOI: 10.1126/science.290.5499.2077] |Summary »|Full Text »
RESEARCH ARTICLES
J. L. Riechmann, J. Heard, G. Martin, L. Reuber, C. -Z., Jiang, J. Keddie, L. Adam, O. Pineda, O. J. Ratcliffe, R. R. Samaha, R. Creelman, M. Pilgrim, P. Broun, J. Z. Zhang, D. Ghandehari, B. K. Sherman, and G. -L. Yu (15 December 2000) Science290 (5499), 2105.
[DOI: 10.1126/science.290.5499.2105] |Abstract »|Full Text »|PDF »|Supplemental Data »
RESEARCH ARTICLES
Todd J. Vision, Daniel G. Brown, and Steven D. Tanksley (15 December 2000) Science290 (5499), 2114.
[DOI: 10.1126/science.290.5499.2114] |Abstract »|Full Text »|PDF »
