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Science 11 December 1998:
Vol. 282. no. 5396, pp. 2095 - 2098
DOI: 10.1126/science.282.5396.2095
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Reports

Eight Calves Cloned from Somatic Cells of a Single Adult

Yoko Kato, Tetsuya Tani, Yusuke Sotomaru, Kazuo Kurokawa, Jun-ya Kato, Hiroshi Doguchi, Hiroshi Yasue, Yukio Tsunoda *

Eight calves were derived from differentiated cells of a single adult cow, five from cumulus cells and three from oviductal cells out of 10 embryos transferred to surrogate cows (80 percent success). All calves were visibly normal, but four died at or soon after birth from environmental causes, and postmortem analysis revealed no abnormality. These results show that bovine cumulus and oviductal epithelial cells of the adult have the genetic content to direct the development of newborn calves.

Y. Kato, Y. Sotomaru, Y. Tsunoda, Laboratory of Animal Reproduction, College of Agriculture and Research Institute for Animal Developmental Biotechnology, Kinki University, 3327-204, Nakamachi, Nara, 631-8505, Japan. T. Tani, Laboratory of Animal Reproduction, College of Agriculture, Kinki University, 3327-204, Nakamachi, Nara, 631-8505, Japan. K. Kurokawa and J. Kato, Division of Molecular Oncology, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara, 630-0101, Japan. H. Doguchi and H. Yasue, Department of Animal Breeding and Genetics, National Institute of Animal Industry, Ministry of Agriculture, Forestry, and Fisheries (MAFF), Tsukuba, Ibaraki 305-0901 Japan.
*   To whom correspondence should be addressed. E-mail: tsunoda{at}nara.kindai.ac.jp

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Biol Reprod 62, 1135-1140
   Abstract »    Full Text »
Extension of Cell Life-Span and Telomere Length in Animals Cloned from Senescent Somatic Cells.
R. P. Lanza, J. B. Cibelli, C. Blackwell, V. J. Cristofalo, M. K. Francis, G. M. Baerlocher, J. Mak, M. Schertzer, E. A. Chavez, N. Sawyer, et al. (2000)
Science 288, 665-669
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Analysis of Gene Transcription in Bovine Nuclear Transfer Embryos Reconstructed with Granulosa Cell Nuclei.
R. Daniels, V. Hall, and A.O. Trounson (2000)
Biol Reprod 63, 1034-1040
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Epigenetic Modifications Necessary for Normal Development Are Established During Oocyte Growth in Mice.
S. Bao, Y. Obata, J. Carroll, I. Domeki, and T. Kono (2000)
Biol Reprod 62, 616-621
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From the Cover: Six cloned calves produced from adult fibroblast cells after long-term culture.
C. Kubota, H. Yamakuchi, J. Todoroki, K. Mizoshita, N. Tabara, M. Barber, and X. Yang (2000)
PNAS 97, 990-995
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Cell Cycle Synchronization of Porcine Fetal Fibroblasts: Effects of Serum Deprivation and Reversible Cell Cycle Inhibitors.
W.A. Kues, M. Anger, J.W. Carnwath, D. Paul, J. Motlik, and H. Niemann (2000)
Biol Reprod 62, 412-419
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Production of Live Calves Derived from Embryonic Stem-Like Cells Aggregated with Tetraploid Embryos.
S. Iwasaki, K. H.S. Campbell, C. Galli, K. Akiyama, and S. Iwasaki (2000)
Biol Reprod 62, 470-475
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Clonal Propagation of Primate Offspring by Embryo Splitting.
A. W. Chan, T. Dominko, C. M. Luetjens, E. Neuber, C. Martinovich, L. Hewitson, C. R. Simerly, and G. P. Schatten (2000)
Science 287, 317-319
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Reprogramming nuclei: insights from cloning, nuclear transfer and heterokaryons.
N Kikyo and A. Wolffe (2000)
J. Cell Sci. 113, 11-20
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