Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
Joseph L. DeRisi,
Vishwanath R. Iyer,
Patrick O. Brown
*
DNA microarrays containing virtually every gene of
Saccharomyces cerevisiae were used to carry out a
comprehensive investigation of the temporal program of gene expression
accompanying the metabolic shift from fermentation to respiration. The
expression profiles observed for genes with known metabolic functions
pointed to features of the metabolic reprogramming that occur during
the diauxic shift, and the expression patterns of many previously
uncharacterized genes provided clues to their possible functions. The
same DNA microarrays were also used to identify genes whose expression was affected by deletion of the transcriptional co-repressor
TUP1 or overexpression of the transcriptional activator
YAP1. These results demonstrate the feasibility and utility
of this approach to genomewide exploration of gene expression patterns.
Department of Biochemistry, Stanford University School of
Medicine, Howard Hughes Medical Institute, Stanford, CA 94305-5428,
USA.
*
To whom correspondence should be addressed. E-mail:
pbrown{at}cmgm.stanford.edu
