Motoaki Seki,12
Mari Narusaka,1
Asako Kamiya,1
Junko Ishida,1
Masakazu Satou,1
Tetsuya Sakurai,1
Maiko Nakajima,1
Akiko Enju,1
Kenji Akiyama,1
Youko Oono,23
Masami Muramatsu,45
Yoshihide Hayashizaki,45
Jun Kawai,45
Piero Carninci,45
Masayoshi Itoh,45
Yoshiyuki Ishii,45
Takahiro Arakawa,45
Kazuhiro Shibata,45
Akira Shinagawa,45
Kazuo Shinozaki12*
Full-length complementary DNAs (cDNAs) are essential for the
correct annotation of genomic sequences and for the functional analysis
of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end
expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were
clustered into 14,668 nonredundant cDNA groups, about 60% of predicted
genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL
cDNAs is useful for promoter analysis and correct annotation of
predicted transcription units and gene products. Furthermore, the
full-length cDNAs are useful resources for analyses of the expression
profiles, functions, and structures of plant proteins.
1 Plant Mutation Exploration Team, Plant
Functional Genomics Research Group, RIKEN Genomic Sciences Center
(GSC), 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
2 Laboratory of Plant Molecular Biology, RIKEN
Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
3 Master's Program in Biosystem Studies, University
of Tsukuba, Tennoudai, Tsukuba, Ibaraki, 305-0074, Japan.
4 Laboratory for Genome Exploration Research Group,
RIKEN GSC, Yokohama Institute 1-7-22 Suehiro-cho, Tsurumi-ku, Yokokama,
Kanagawa, 230-0045, Japan.
5 Genome Science
Laboratory, RIKEN, 2-1 Hirosawa, Wako, 351-0198, Japan.
*
To whom correspondence should be addressed. Laboratory of Plant
Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba
305-0074, Japan. E-mail: sinozaki{at}rtc.riken.go.jp
