Bone Augmentation in Rat by Highly Porous β-TCP Scaffolds with Different Open-cell Sizes in Combination with Fibroblast Growth Factor-2

Hirofumi MIYAJI; Hiroyuki YOKOYAMA; Yuta KOSEN; Hiroyuki NISHIMURA; Kazuyasu NAKANE; Saori TANAKA; Kaori OTANI; Kana INOUE; Asako IBARA; Izumi KANAYAMA; Takashi YOSHIDA; Kosuke OGAWA; Erika NISHIDA; Masamitsu KAWANAMI

doi:10.11223/jarde.10.172

ORIGINAL ARTICLES

Bone Augmentation in Rat by Highly Porous β-TCP Scaffolds with Different Open-cell Sizes in Combination with Fibroblast Growth Factor-2

Hirofumi MIYAJI, Hiroyuki YOKOYAMA, Yuta KOSEN, Hiroyuki NISHIMURA, Kazuyasu NAKANE, Saori TANAKA, Kaori OTANI, Kana INOUE, Asako IBARA, Izumi KANAYAMA, Takashi YOSHIDA, Kosuke OGAWA, Erika NISHIDA, Masamitsu KAWANAMI

Author information

Hirofumi MIYAJI
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Hiroyuki YOKOYAMA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Yuta KOSEN
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Hiroyuki NISHIMURA
Inoac Corporation, Nagoya, Japan
Kazuyasu NAKANE
Inoac Corporation, Nagoya, Japan
Saori TANAKA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Kaori OTANI
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Kana INOUE
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Asako IBARA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Izumi KANAYAMA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Takashi YOSHIDA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Kosuke OGAWA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Erika NISHIDA
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
Masamitsu KAWANAMI
Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan

Keywords: bone tissue engineering, beta-tricalcium phosphate (β-TCP), regenerative scaffold, fibroblast growth factor-2 (FGF2), open-cell structure

JOURNAL FREE ACCESS

2013 Volume 10 Issue 3 Pages 172-181

DOI https://doi.org/10.11223/jarde.10.172

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Abstract

We prepared highly porous beta-tricalcium phosphate (β-TCP) scaffolds with different open-cell structure sizes. The aim of this study was to examine whether the open-cell size of the scaffold affected osteoinduction in combination with fibroblast growth factor-2 (FGF2) in rats. Polyurethane foam was immersed in β-TCP slurry and sintered in a furnace. Porous β-TCP scaffolds were prepared in three cell sizes (0.6, 0.4 and 0.3 mm) and characterized. Subsequently, each scaffold with FGF2 was implanted to rat cranial bone. Histomorphometric analyses were taken at 35 days post-surgery. The results showed that each β-TCP scaffold exhibited fully interconnected porosity, and frequently allowed bone tissue ingrowth. The 0.4-mm cell sized scaffold significantly promoted bone augmentation compared to the 0.3-mm type. Resorption of the β-TCP scaffold of 0.4-mm cell size was frequently accelerated. In conclusion, FGF2-loaded β-TCP scaffolds with 0.4-mm cell size would be effective for bone tissue engineering.

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