Fourier Transform Methods Applied To The Analysis Of Microphotometric Images Of Histologic Sections

Harvey E. Dytch; George L. Wied

doi:10.1117/12.951889

13 June 1989 Fourier Transform Methods Applied To The Analysis Of Microphotometric Images Of Histologic Sections

Harvey E. Dytch, George L. Wied

Proceedings Volume 1063, New Technologies in Cytometry; (1989) https://doi.org/10.1117/12.951889
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

Implementation of one-dimensional Fourier transform techniques for the analysis of histologic sections is discussed, as is the motivation for their use. Features of the frequency domain representation derived by such a transform are shown to be related to several important diagnostic clues. The interpretation of the Fourier magnitude spectrum in histologically relevant terms is examined by means of Fourier transforms of idealized tissue simulations. Some of the perturbations of these ideal spectra produced by biologic reality are discussed. Three classic types of cervical epithelial tissue are modeled, and their representation as Fourier magnitude spectra interpreted in the light of the previous results: characteristic frequency domain signatures are obtained for each. It is concluded that these techniques may provide diagnostically important objective measures, and may be applied to otherwise intractable histologic specimens with crowded and overlapping nuclei.

Citation Download Citation

Harvey E. Dytch and George L. Wied "Fourier Transform Methods Applied To The Analysis Of Microphotometric Images Of Histologic Sections", Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); https://doi.org/10.1117/12.951889

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