A Scanning Laser Microscope For Muscle Fiber Studies

Ian Hunter; Serge Lafontaine; Peter Hunter

doi:10.1117/12.941511

3 August 1987 A Scanning Laser Microscope For Muscle Fiber Studies

Ian Hunter, Serge Lafontaine, Peter Hunter

Proceedings Volume 0809, Scanning Imaging Technology; (1987) https://doi.org/10.1117/12.941511
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

A scanning laser microscope (SLM) has been constructed for use in imaging single living skeletal muscle fibers. The muscle fiber is kept alive and stimulated in a temperature-controlled chamber through which a nutrient solution flows. The fiber is attached at each end to force and displacement transducers which are in turn connected to electromagnetic (voice-coil type) linear motors. These motors scan the fiber in one axis (x-axis) and are also used to stretch and shorten the fiber during mechanical experiments. Each of the x-axis linear motors is in turn mounted on a micro-stepping motor which provides for rotation (r-axis) and twist of the muscle fiber. A laser beam is focused at a location within the fiber by high numerical aperture confocal optics which form one path of a mach-Zehnder interferometer. The position of the muscle fiber with respect to the laser beam may be further manipulated by two additional electromagnetic linear motors (y-and z-axis) to permit 3-linear and 1-rotary axis scanning. The scanning pattern is controlled by a Micro-VAX-II computer which is also used to sample the output of the photo-detector and the force and displacement transducers. A complex 3-D image is acquired and processed (e.g. SLM optical transfer function identification and deconvolution) on the VAX using NEXUS-plus a language for linear and nonlinear signal and system analysis.

Citation Download Citation

Ian Hunter, Serge Lafontaine, and Peter Hunter "A Scanning Laser Microscope For Muscle Fiber Studies", Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); https://doi.org/10.1117/12.941511

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