Lung imaging in rodents using dual energy micro-CT

C. T. Badea; X. Guo; D. Clark; S. M. Johnston; C. Marshall; C. Piantadosi

doi:10.1117/12.912155

16 April 2012 Lung imaging in rodents using dual energy micro-CT

C. T. Badea, X. Guo, D. Clark, S. M. Johnston, C. Marshall, C. Piantadosi

Proceedings Volume 8317, Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging; 83171I (2012) https://doi.org/10.1117/12.912155
Event: SPIE Medical Imaging, 2012, San Diego, California, United States

Abstract

Dual energy CT imaging is expected to play a major role in the diagnostic arena as it provides material decomposition on an elemental basis. The purpose of this work is to investigate the use of dual energy micro-CT for the estimation of vascular, tissue, and air fractions in rodent lungs using a post-reconstruction three-material decomposition method. We have tested our method using both simulations and experimental work. Using simulations, we have estimated the accuracy limits of the decomposition for realistic micro-CT noise levels. Next, we performed experiments involving ex vivo lung imaging in which intact lungs were carefully removed from the thorax, were injected with an iodine-based contrast agent and inflated with air at different volume levels. Finally, we performed in vivo imaging studies in (n=5) C57BL/6 mice using fast prospective respiratory gating in endinspiration and end-expiration for three different levels of positive end-expiratory pressure (PEEP). Prior to imaging, mice were injected with a liposomal blood pool contrast agent. The mean accuracy values were for Air (95.5%), Blood (96%), and Tissue (92.4%). The absolute accuracy in determining all fraction materials was 94.6%. The minimum difference that we could detect in material fractions was 15%. As expected, an increase in PEEP levels for the living mouse resulted in statistically significant increases in air fractions at end-expiration, but no significant changes in end-inspiration. Our method has applicability in preclinical pulmonary studies where various physiological changes can occur as a result of genetic changes, lung disease, or drug effects.

Citation Download Citation

C. T. Badea, X. Guo, D. Clark, S. M. Johnston, C. Marshall, and C. Piantadosi "Lung imaging in rodents using dual energy micro-CT", Proc. SPIE 8317, Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging, 83171I (16 April 2012); https://doi.org/10.1117/12.912155

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