Silicon cell culture templates with nanotopography: periodic nanostructures and random nanoporous topologies generated by high-repetition rate sub-15 fs pulsed near-infrared laser light

Martin Straub; Aisada Uchugonova; Marcus Koch; Karsten König

doi:10.1117/12.905012

2 February 2012 Silicon cell culture templates with nanotopography: periodic nanostructures and random nanoporous topologies generated by high-repetition rate sub-15 fs pulsed near-infrared laser light

Martin Straub, Aisada Uchugonova, Marcus Koch, Karsten König

Author Affiliations +

Proceedings Volume 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 82310P (2012) https://doi.org/10.1117/12.905012
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

In recent years a variety of studies has demonstrated that artificially generated microenvironments can exert a strong influence on cell growth, cell adhesion, proliferation, and differentiation behavior in the culture dish. In particular, cells tend to adapt themselves to elongated micro- and nanostructures. Thus, nanostructured substrates are of significant interest in the biological and biomedical sciences as adhesion and development of cells can be controlled via the topological surface properties. In contrast to earlier approaches relying on electron beam or nanoimprint lithography, nanostructures were produced on Si(100) surfaces using sub-15 femtosecond high-resolution laser scanning microscopy. Laser processing was performed with the silicon surface immersed in water followed by hydrofluoric acid etching in order to remove silicon oxide residues. Ripples of at a periodicity of 150 nm as well as random nanoporous surface arrangements were generated by Ti:Sapphire laser light of centre wavelength 800 nm (bandwidth 120 nm, repetition rate 85 MHz) at picojoule pulse energies. Growth of Chinese hamster ovary (CHO) cells revealed good adhesion to the silicon substrates. Importantly, alignment of cells along the direction of ripples was observed, whereas randomly nanoporous surfaces did not induce any preferences in cell orientation.

Citation Download Citation

Martin Straub, Aisada Uchugonova, Marcus Koch, and Karsten König "Silicon cell culture templates with nanotopography: periodic nanostructures and random nanoporous topologies generated by high-repetition rate sub-15 fs pulsed near-infrared laser light", Proc. SPIE 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 82310P (2 February 2012); https://doi.org/10.1117/12.905012

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