A simple mixture to enhance muscle transmittance

Luís Oliveira; Armindo Lage; Manuel Pais Clemente; Valery V. Tuchin

doi:10.1117/12.803978

9 June 2008 A simple mixture to enhance muscle transmittance

Luís Oliveira, Armindo Lage, Manuel Pais Clemente, Valery V. Tuchin

Proceedings Volume 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX; 67910J (2008) https://doi.org/10.1117/12.803978
Event: Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 2006, Saratov, Russian Federation

Abstract

Skeletal muscle is a fibrous tissue composed by muscle fibers and interstitial fluid. Due to this constitution, the muscle presents a non uniform refractive index profile that origins strong light scattering. One way to improve tissue transmittance is to reduce this refractive index mismatch by immersing the muscle in an optical clearing agent. As a consequence of such immersion tissue also suffers dehydration. The study of the optical clearing effect created by a simple mixture composed by ethanol, glycerol and distilled water has proven its effectiveness according to the variations observed in the parameters under study. The effect was characterized in terms of its magnitude, time duration and histological variations. The applied treatment has created a small reduction of the global sample refractive index that is justified by the long time rehydration caused by water in the immersing solution. From the reduction in sample pH we could also identify the dehydration process created in the sample. The immersion treatment has originated fiber bundle contraction and a spread distribution of the muscle fiber bundles inside. New studies with the mixture used, or with other combinations of its constituents might be interesting to perform with the objective to develop new clinical procedures.

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Luís Oliveira, Armindo Lage, Manuel Pais Clemente, and Valery V. Tuchin "A simple mixture to enhance muscle transmittance", Proc. SPIE 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 67910J (9 June 2008); https://doi.org/10.1117/12.803978

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KEYWORDS

Refractive index

Transmittance

Tissue optics

Tissues

Optical fibers

Optical clearing

Photography

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