Fluorescence and absorbance measurements for chromatographic analysis using a miniaturized micro-optical detection unit

Sara Van Overmeire; Heidi Ottevaere; Gert Desmet; Hugo Thienpont

doi:10.1117/12.780490

2 May 2008 Fluorescence and absorbance measurements for chromatographic analysis using a miniaturized micro-optical detection unit

Sara Van Overmeire, Heidi Ottevaere, Gert Desmet, Hugo Thienpont

Author Affiliations +

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69910V (2008) https://doi.org/10.1117/12.780490
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

We present a micro-optical detection unit for both laser induced fluorescence and absorbance analysis in fused silica capillaries, which can be used for chromatographic applications. The detection system is designed by means of non-sequential ray tracing simulations and prototyped by means of Deep Proton Writing. Such a prototyped master component is afterwards replicated by means of elastomeric moulding and vacuum casting. In a proof-of-concept demonstration the prototyped master micro-optical unit is used for the detection of various concentrations of coumarin dyes. The detection limit (SNR=3.3) achieved measures 0.6nM for fluorescence analysis and 12μM for absorbance measurements in capillaries with an inner diameter of 150μm. We discuss the optimization of different measurement parameters of the detectors in the setup in order to achieve accurate, fast and sensitive measurements.

Citation Download Citation

Sara Van Overmeire, Heidi Ottevaere, Gert Desmet, and Hugo Thienpont "Fluorescence and absorbance measurements for chromatographic analysis using a miniaturized micro-optical detection unit", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910V (2 May 2008); https://doi.org/10.1117/12.780490

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