Enlarging the linear response range of velocity with optimum imaging parameters and modified data processing in laser speckle imaging

Jianjun Qiu; Pengcheng Li; Sergey S. Ul'yanov; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.763355

28 February 2008 Enlarging the linear response range of velocity with optimum imaging parameters and modified data processing in laser speckle imaging

Jianjun Qiu, Pengcheng Li, Sergey S. Ul'yanov, Shaoqun Zeng, Qingming Luo

Author Affiliations +

Proceedings Volume 6863, Optical Diagnostics and Sensing VIII; 68630T (2008) https://doi.org/10.1117/12.763355
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Laser speckle imaging (LSI) technique is considered as a promising method of accessing cerebral blood flow (CBF) of animals for its high spatiotemporal resolution and simplicity. It is important in LSI that optimum imaging parameters and limited noises should be confirmed to promote the imaging precision. We investigated in this paper different factors which may affect the imaging results with a moving white plate model, and then proposed a method of enlarging the linear response range of velocity. Through experiment, we proposed in our LSI system the optimum imaging parameters, including the numerical aperture and magnification of microscopy, the integration time, the gain mode of CCD camera. The average intensity was found optimum at about 800 counts out of 4096 grey level, which permits the highest contrast in our experiment. To eliminate the influence of uneven illumination, a direct current weight of 27 counts was subtracted during data processing. The result indicated that the relationship between measured velocity and the real one remained linear with R² equaling to 0.99 throughout the scale of 80 mm/s.

Citation Download Citation

Jianjun Qiu, Pengcheng Li, Sergey S. Ul'yanov, Shaoqun Zeng, and Qingming Luo "Enlarging the linear response range of velocity with optimum imaging parameters and modified data processing in laser speckle imaging", Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 68630T (28 February 2008); https://doi.org/10.1117/12.763355

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available