In vivo visualizing the dynamics of bone marrow stem cells in mouse retina and choroidal-retinal circulation

Heuy-Ching H. Wang; Harry Zwick; Peter R. Edsall; Rachel D. Cheramie; David J. Lund; Bruce Stuck

doi:10.1117/12.711487

5 March 2007 In vivo visualizing the dynamics of bone marrow stem cells in mouse retina and choroidal-retinal circulation

Heuy-Ching H. Wang, Harry Zwick, Peter R. Edsall, Rachel D. Cheramie, David J. Lund, Bruce Stuck

Author Affiliations +

Proceedings Volume 6426, Ophthalmic Technologies XVII; 642621 (2007) https://doi.org/10.1117/12.711487
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

It has recently been shown that bone marrow cells can differentiate into various lineage cells including neural cells in vitro and in vivo. Therefore it is an attractive therapeutic intervention to apply autologous bone marrow-derived stem cells that may offer neuroprotection to laser-induced retinal injuries. The purpose of this study is to develop a method with which to visualize bone marrow stem cells dynamics in mouse retinal circulation. We have used a physiological method, confocal scanning laser ophthalmoscope (SLO), to track the highly enriched stem/progenitor cells circulating in the retina. Stem cells were enriched by immunomagnetic depletion of cells committed to the T- and B lymphocytic, myeloid and erythorid lineages. CellTracker^TM Green-labeled stem cells were injected into the tail veins of mice with laser-induced focal retinal injuries. Bone marrow stem cells labeled with CellTracker^TM Green were visible in the retinal circulation for as long as 1 hour and 30 minutes. These studies suggest that stem cell-enriched bone marrow cells may have the ability to mobilize into laser-induced retinal injuries and possibly further proliferate, differentiate and functionally integrate into the retina.

Citation Download Citation

Heuy-Ching H. Wang, Harry Zwick, Peter R. Edsall, Rachel D. Cheramie, David J. Lund, and Bruce Stuck "In vivo visualizing the dynamics of bone marrow stem cells in mouse retina and choroidal-retinal circulation", Proc. SPIE 6426, Ophthalmic Technologies XVII, 642621 (5 March 2007); https://doi.org/10.1117/12.711487

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